Detailed information of TA system TA227331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4946227..4947908
• Replicon: chromosome
• Accession: NZ_CP089001
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A6TH06
• Locus tag: LRZ77_RS24370
• Protein ID: WP_015959269.1
• Coordinates: 4946227..4947102 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LRZ77_RS24375
• Protein ID: WP_004177809.1
• Coordinates: 4947150..4947908 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS24350 (4941533)	4941533..4943053	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LRZ77_RS24355 (4943050)	4943050..4944048	+	999	WP_002885172.1	ribose ABC transporter permease	-
LRZ77_RS24360 (4944079)	4944079..4945020	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LRZ77_RS24365 (4945095)	4945095..4946102	+	1008	WP_015959270.1	carbohydrate kinase family protein	-
LRZ77_RS24370 (4946227)	4946227..4947102	-	876	WP_015959269.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LRZ77_RS24375 (4947150)	4947150..4947908	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LRZ77_RS24380 (4947994)	4947994..4948722	-	729	WP_032413197.1	response regulator transcription factor	-
LRZ77_RS24385 (4948995)	4948995..4951142	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
LRZ77_RS24390 (4951139)	4951139..4951282	-	144	WP_162897205.1	hypothetical protein	-
LRZ77_RS24395 (4951720)	4951720..4952406	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32990.17 Da
• Isoelectric Point: 5.4637

>T227331 WP_015959269.1 NZ_CP089001:c4947102-4946227 [Klebsiella pneumoniae]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQAGMSAVLQANRSDE
MRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKSCITQQDVDFLLSFK
RGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T227331 NZ_CP122837:3528559-3528777 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT227331 WP_004177809.1 NZ_CP089001:c4947908-4947150 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT227331 NZ_CP122837:3528159-3528533 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A6TH06

Antitoxin

• AlphaFold DB: A6TH05