Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4657089..4657899 Replicon chromosome
Accession NZ_CP089001
Organism Klebsiella pneumoniae strain 56661

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LRZ77_RS22940 Protein ID WP_002887280.1
Coordinates 4657089..4657622 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LRZ77_RS22945 Protein ID WP_002887278.1
Coordinates 4657633..4657899 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ77_RS22935 (4655921) 4655921..4657042 + 1122 WP_009309849.1 cupin domain-containing protein -
LRZ77_RS22940 (4657089) 4657089..4657622 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LRZ77_RS22945 (4657633) 4657633..4657899 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LRZ77_RS22950 (4658002) 4658002..4659435 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
LRZ77_RS22955 (4659425) 4659425..4660108 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
LRZ77_RS22960 (4660281) 4660281..4661666 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
LRZ77_RS22965 (4661684) 4661684..4661989 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T227328 WP_002887280.1 NZ_CP089001:c4657622-4657089 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T227328 NZ_CP122837:2761470-2761577 [Escherichia coli]
ATGACGCTCGCGCAGTTTACCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT227328 WP_002887278.1 NZ_CP089001:c4657899-4657633 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT227328 NZ_CP122837:c2761423-2761357 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References