227327

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4029272..4029891 Replicon chromosome
Accession NZ_CP089001
Organism Klebsiella pneumoniae strain 56661

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ77_RS19945 Protein ID WP_002892050.1
Coordinates 4029673..4029891 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ77_RS19940 Protein ID WP_002892066.1
Coordinates 4029272..4029646 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ77_RS19930 (4024424) 4024424..4025617 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ77_RS19935 (4025640) 4025640..4028786 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ77_RS19940 (4029272) 4029272..4029646 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ77_RS19945 (4029673) 4029673..4029891 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ77_RS19950 (4030054) 4030054..4030620 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ77_RS19955 (4030592) 4030592..4030732 - 141 WP_004147370.1 hypothetical protein -
LRZ77_RS19960 (4030753) 4030753..4031223 + 471 WP_130948794.1 YlaC family protein -
LRZ77_RS19965 (4031198) 4031198..4032649 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
LRZ77_RS19970 (4032750) 4032750..4033448 + 699 WP_023287311.1 GNAT family protein -
LRZ77_RS19975 (4033445) 4033445..4033585 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ77_RS19980 (4033585) 4033585..4033848 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227327 WP_002892050.1 NZ_CP089001:4029673-4029891 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227327 NZ_CP122837:c2561269-2561093 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227327 WP_002892066.1 NZ_CP089001:4029272-4029646 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227327 NZ_CP122837:c2561047-2560631 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGTGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References