Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1474878..1475614 Replicon chromosome
Accession NZ_CP089001
Organism Klebsiella pneumoniae strain 56661

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag LRZ77_RS07285 Protein ID WP_032433360.1
Coordinates 1475132..1475614 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LRZ77_RS07280 Protein ID WP_003026799.1
Coordinates 1474878..1475144 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ77_RS07255 (1470524) 1470524..1471663 + 1140 WP_032433368.1 mannitol dehydrogenase -
LRZ77_RS07260 (1471692) 1471692..1472354 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
LRZ77_RS07265 (1472338) 1472338..1473342 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
LRZ77_RS07270 (1473360) 1473360..1473992 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
LRZ77_RS07275 (1474002) 1474002..1474565 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
LRZ77_RS07280 (1474878) 1474878..1475144 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LRZ77_RS07285 (1475132) 1475132..1475614 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
LRZ77_RS07290 (1475821) 1475821..1477167 + 1347 WP_077253535.1 ISNCY family transposase -
LRZ77_RS07295 (1477216) 1477216..1477611 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
LRZ77_RS07300 (1477962) 1477962..1478561 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
LRZ77_RS07305 (1478774) 1478774..1479718 - 945 WP_077254249.1 fimbrial protein -
LRZ77_RS07310 (1479730) 1479730..1480308 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1442047..1488544 46497
- flank IS/Tn - - 1475809..1477167 1358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T227317 WP_032433360.1 NZ_CP089001:1475132-1475614 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T227317 NZ_CP122835:36669-37085 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT227317 WP_003026799.1 NZ_CP089001:1474878-1475144 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227317 NZ_CP122835:36442-36672 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References