Detailed information of TA system TA227317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1474878..1475614
• Replicon: chromosome
• Accession: NZ_CP089001
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A4S4Y1H2
• Locus tag: LRZ77_RS07285
• Protein ID: WP_032433360.1
• Coordinates: 1475132..1475614 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LRZ77_RS07280
• Protein ID: WP_003026799.1
• Coordinates: 1474878..1475144 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS07255 (1470524)	1470524..1471663	+	1140	WP_032433368.1	mannitol dehydrogenase	-
LRZ77_RS07260 (1471692)	1471692..1472354	+	663	WP_032433366.1	dihydroxyacetone kinase subunit DhaL	-
LRZ77_RS07265 (1472338)	1472338..1473342	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
LRZ77_RS07270 (1473360)	1473360..1473992	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
LRZ77_RS07275 (1474002)	1474002..1474565	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
LRZ77_RS07280 (1474878)	1474878..1475144	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LRZ77_RS07285 (1475132)	1475132..1475614	+	483	WP_032433360.1	GNAT family N-acetyltransferase	Toxin
LRZ77_RS07290 (1475821)	1475821..1477167	+	1347	WP_077253535.1	ISNCY family transposase	-
LRZ77_RS07295 (1477216)	1477216..1477611	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
LRZ77_RS07300 (1477962)	1477962..1478561	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
LRZ77_RS07305 (1478774)	1478774..1479718	-	945	WP_077254249.1	fimbrial protein	-
LRZ77_RS07310 (1479730)	1479730..1480308	-	579	WP_032432061.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1442047..1488544	46497
	-	flank	IS/Tn	-	-	1475809..1477167	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17421.02 Da
• Isoelectric Point: 9.6047

>T227317 WP_032433360.1 NZ_CP089001:1475132-1475614 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T227317 NZ_CP122835:36669-37085 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT227317 WP_003026799.1 NZ_CP089001:1474878-1475144 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT227317 NZ_CP122835:36442-36672 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A4S4Y1H2

Antitoxin

• AlphaFold DB: A0A1Q8YL66