Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 18826..19469 | Replicon | plasmid p49088-116.5 |
| Accession | NZ_CP088999 | ||
| Organism | Klebsiella pneumoniae strain 49088 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | LRZ82_RS27015 | Protein ID | WP_001044770.1 |
| Coordinates | 19053..19469 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | LRZ82_RS27010 | Protein ID | WP_001261282.1 |
| Coordinates | 18826..19056 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRZ82_RS26985 (LRZ82_26985) | 14766..14933 | - | 168 | Protein_17 | IS1 family transposase | - |
| LRZ82_RS26990 (LRZ82_26990) | 15237..16166 | + | 930 | WP_004213558.1 | Rpn family recombination-promoting nuclease/putative transposase | - |
| LRZ82_RS26995 (LRZ82_26995) | 16311..17090 | - | 780 | WP_004213560.1 | site-specific integrase | - |
| LRZ82_RS27000 (LRZ82_27000) | 17087..17908 | - | 822 | WP_004213562.1 | hypothetical protein | - |
| LRZ82_RS27005 (LRZ82_27005) | 18453..18869 | - | 417 | WP_164481821.1 | hypothetical protein | - |
| LRZ82_RS27010 (LRZ82_27010) | 18826..19056 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LRZ82_RS27015 (LRZ82_27015) | 19053..19469 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LRZ82_RS27020 (LRZ82_27020) | 19543..21105 | + | 1563 | WP_004206609.1 | AAA family ATPase | - |
| LRZ82_RS27025 (LRZ82_27025) | 21090..22112 | + | 1023 | WP_000361404.1 | helicase UvrD | - |
| LRZ82_RS27030 (LRZ82_27030) | 22368..23064 | + | 697 | WP_024269734.1 | IS1 family transposase | - |
| LRZ82_RS27035 (LRZ82_27035) | 23093..23365 | + | 273 | Protein_27 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..116545 | 116545 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T227306 WP_001044770.1 NZ_CP088999:19053-19469 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T227306 NZ_CP122832:2889111-2889218 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT227306 WP_001261282.1 NZ_CP088999:18826-19056 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT227306 NZ_CP122832:c2889064-2888998 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |