Detailed information of TA system TA227305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2794..3268
• Replicon: plasmid p49088-6.796
• Accession: NZ_CP088998
• Organism: Klebsiella pneumoniae strain 49088

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LRZ82_RS26875
• Protein ID: WP_004197762.1
• Coordinates: 3002..3268 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LRZ82_RS26870
• Protein ID: WP_000879771.1
• Coordinates: 2794..3018 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ82_RS26845 (LRZ82_26845)	211..387	+	177	WP_004197757.1	hypothetical protein	-
LRZ82_RS26850 (LRZ82_26850)	377..721	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LRZ82_RS26855 (LRZ82_26855)	930..1364	+	435	Protein_2	relaxase/mobilization nuclease domain-containing protein	-
LRZ82_RS26860 (LRZ82_26860)	1399..1908	+	510	WP_032433972.1	MbeB family mobilization protein	-
LRZ82_RS26865 (LRZ82_26865)	1915..2127	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LRZ82_RS26870 (LRZ82_26870)	2794..3018	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LRZ82_RS26875 (LRZ82_26875)	3002..3268	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRZ82_RS26880 (LRZ82_26880)	3338..3688	-	351	WP_004197756.1	hypothetical protein	-
LRZ82_RS26885 (LRZ82_26885)	3754..3942	-	189	WP_228264513.1	hypothetical protein	-
LRZ82_RS26890 (LRZ82_26890)	4102..5082	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
LRZ82_RS26895 (LRZ82_26895)	5128..5499	-	372	WP_228264514.1	hypothetical protein	-
LRZ82_RS26900 (LRZ82_26900)	5525..5851	-	327	WP_050516389.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6796	6796
	-	flank	IS/Tn	-	-	4102..5082	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T227305 WP_004197762.1 NZ_CP088998:3002-3268 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T227305 NZ_CP122832:c2578284-2578108 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT227305 WP_000879771.1 NZ_CP088998:2794-3018 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT227305 NZ_CP122832:c2578062-2577646 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TGCTCACCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5