Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5359616..5360241 Replicon chromosome
Accession NZ_CP088997
Organism Klebsiella pneumoniae strain 49088

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRZ82_RS26480 Protein ID WP_002882817.1
Coordinates 5359616..5359999 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRZ82_RS26485 Protein ID WP_004150355.1
Coordinates 5359999..5360241 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ82_RS26465 (LRZ82_26465) 5356982..5357884 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRZ82_RS26470 (LRZ82_26470) 5357881..5358516 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRZ82_RS26475 (LRZ82_26475) 5358513..5359442 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRZ82_RS26480 (LRZ82_26480) 5359616..5359999 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ82_RS26485 (LRZ82_26485) 5359999..5360241 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRZ82_RS26490 (LRZ82_26490) 5360446..5361363 + 918 WP_002882812.1 alpha/beta hydrolase -
LRZ82_RS26495 (LRZ82_26495) 5361377..5362318 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRZ82_RS26500 (LRZ82_26500) 5362363..5362800 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRZ82_RS26505 (LRZ82_26505) 5362797..5363657 - 861 WP_002882807.1 virulence factor BrkB family protein -
LRZ82_RS26510 (LRZ82_26510) 5363651..5364250 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227304 WP_002882817.1 NZ_CP088997:c5359999-5359616 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227304 NZ_CP122832:2099656-2099759 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227304 WP_004150355.1 NZ_CP088997:c5360241-5359999 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227304 NZ_CP122832:c2099797-2099526 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References