Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4163400..4164019 Replicon chromosome
Accession NZ_CP088997
Organism Klebsiella pneumoniae strain 49088

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ82_RS20740 Protein ID WP_002892050.1
Coordinates 4163801..4164019 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ82_RS20735 Protein ID WP_002892066.1
Coordinates 4163400..4163774 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ82_RS20725 (LRZ82_20725) 4158552..4159745 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ82_RS20730 (LRZ82_20730) 4159768..4162914 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ82_RS20735 (LRZ82_20735) 4163400..4163774 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ82_RS20740 (LRZ82_20740) 4163801..4164019 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ82_RS20745 (LRZ82_20745) 4164178..4164744 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ82_RS20750 (LRZ82_20750) 4164716..4164856 - 141 WP_004147370.1 hypothetical protein -
LRZ82_RS20755 (LRZ82_20755) 4164877..4165347 + 471 WP_002892026.1 YlaC family protein -
LRZ82_RS20760 (LRZ82_20760) 4165322..4166773 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LRZ82_RS20765 (LRZ82_20765) 4166874..4167572 + 699 WP_002892021.1 GNAT family protein -
LRZ82_RS20770 (LRZ82_20770) 4167569..4167709 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ82_RS20775 (LRZ82_20775) 4167709..4167972 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227300 WP_002892050.1 NZ_CP088997:4163801-4164019 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227300 NZ_CP122832:707442-707756 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227300 WP_002892066.1 NZ_CP088997:4163400-4163774 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227300 NZ_CP122832:707753-708169 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References