Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1741312..1741950 Replicon chromosome
Accession NC_010498
Organism Escherichia coli SMS-3-5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ECSMS35_RS08770 Protein ID WP_000813794.1
Coordinates 1741774..1741950 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ECSMS35_RS08765 Protein ID WP_001270286.1
Coordinates 1741312..1741728 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSMS35_RS08745 1736464..1737405 - 942 WP_001251303.1 ABC transporter permease -
ECSMS35_RS08750 1737406..1738419 - 1014 WP_000220391.1 ABC transporter ATP-binding protein -
ECSMS35_RS08755 1738437..1739582 - 1146 WP_000034364.1 ABC transporter substrate-binding protein -
ECSMS35_RS08760 1739827..1741233 - 1407 WP_000760611.1 PLP-dependent aminotransferase family protein -
ECSMS35_RS08765 1741312..1741728 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ECSMS35_RS08770 1741774..1741950 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ECSMS35_RS08775 1742172..1742402 + 231 WP_000491567.1 DUF2554 family protein -
ECSMS35_RS08780 1742494..1744455 - 1962 WP_012311648.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ECSMS35_RS08785 1744528..1745064 - 537 WP_000429154.1 DNA-binding transcriptional regulator SutR -
ECSMS35_RS08790 1745117..1746037 + 921 Protein_1658 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1746052..1747380 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T22730 WP_000813794.1 NC_010498:c1741950-1741774 [Escherichia coli SMS-3-5]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T22730 NC_010498:c1741950-1741774 [Escherichia coli SMS-3-5]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT22730 WP_001270286.1 NC_010498:c1741728-1741312 [Escherichia coli SMS-3-5]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT22730 NC_010498:c1741728-1741312 [Escherichia coli SMS-3-5]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References