Detailed information of TA system TA227286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 143903..144573
• Replicon: plasmid p50700-217.1
• Accession: NZ_CP088996
• Organism: Klebsiella pneumoniae strain 50700

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LRZ81_RS29325
• Protein ID: WP_004213072.1
• Coordinates: 143903..144346 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRZ81_RS29330
• Protein ID: WP_004213073.1
• Coordinates: 144343..144573 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ81_RS29290 (LRZ81_29290)	139314..139589	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRZ81_RS29295 (LRZ81_29295)	139652..140143	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRZ81_RS29300 (LRZ81_29300)	140192..141112	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LRZ81_RS29305 (LRZ81_29305)	141203..141606	+	404	Protein_155	GAF domain-containing protein	-
LRZ81_RS29310 (LRZ81_29310)	142124..142759	-	636	Protein_156	mucoid phenotype regulator RmpA2	-
LRZ81_RS29315 (LRZ81_29315)	143176..143480	+	305	Protein_157	transposase	-
LRZ81_RS29320 (LRZ81_29320)	143503..143754	-	252	WP_186987481.1	hypothetical protein	-
LRZ81_RS29325 (LRZ81_29325)	143903..144346	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ81_RS29330 (LRZ81_29330)	144343..144573	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ81_RS29335 (LRZ81_29335)	145181..146314	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRZ81_RS29340 (LRZ81_29340)	146330..146623	+	294	WP_004213076.1	hypothetical protein	-
LRZ81_RS29345 (LRZ81_29345)	146613..146819	-	207	WP_004213077.1	hypothetical protein	-
LRZ81_RS29350 (LRZ81_29350)	147171..147461	+	291	WP_004213078.1	hypothetical protein	-
LRZ81_RS29355 (LRZ81_29355)	147451..148350	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..217070	217070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227286 WP_004213072.1 NZ_CP088996:c144346-143903 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227286 NZ_CP122823:4151287-4151565 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227286 WP_004213073.1 NZ_CP088996:c144573-144343 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227286 NZ_CP122823:4151024-4151284 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620