Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94117..94760 Replicon plasmid p50700-140.6
Accession NZ_CP088995
Organism Klebsiella pneumoniae strain 50700

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LRZ81_RS28185 Protein ID WP_001044770.1
Coordinates 94117..94533 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LRZ81_RS28190 Protein ID WP_001261282.1
Coordinates 94530..94760 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ81_RS28165 (90220) 90220..90492 - 273 Protein_110 transposase -
LRZ81_RS28175 (91474) 91474..92496 - 1023 WP_000361404.1 helicase UvrD -
LRZ81_RS28180 (92481) 92481..94043 - 1563 WP_004206609.1 AAA family ATPase -
LRZ81_RS28185 (94117) 94117..94533 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ81_RS28190 (94530) 94530..94760 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRZ81_RS28195 (94717) 94717..95178 + 462 WP_014343465.1 hypothetical protein -
LRZ81_RS28200 (95339) 95339..96283 + 945 WP_011977810.1 hypothetical protein -
LRZ81_RS28205 (96320) 96320..96712 + 393 WP_011977811.1 hypothetical protein -
LRZ81_RS28210 (96770) 96770..97291 + 522 WP_013214008.1 hypothetical protein -
LRZ81_RS28215 (97337) 97337..97540 + 204 WP_011977813.1 hypothetical protein -
LRZ81_RS28220 (97570) 97570..98574 + 1005 WP_011977814.1 hypothetical protein -
LRZ81_RS28225 (98758) 98758..99537 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / blaSHV-12 / blaKPC-2 - 1..140550 140550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T227282 WP_001044770.1 NZ_CP088995:c94533-94117 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T227282 NZ_CP122823:3049624-3049731 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT227282 WP_001261282.1 NZ_CP088995:c94760-94530 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT227282 NZ_CP122823:c3049577-3049511 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References