Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5395696..5396321 Replicon chromosome
Accession NZ_CP088991
Organism Klebsiella pneumoniae strain 50700

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRZ81_RS26670 Protein ID WP_002882817.1
Coordinates 5395696..5396079 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRZ81_RS26675 Protein ID WP_004150355.1
Coordinates 5396079..5396321 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ81_RS26655 (LRZ81_26655) 5393062..5393964 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRZ81_RS26660 (LRZ81_26660) 5393961..5394596 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRZ81_RS26665 (LRZ81_26665) 5394593..5395522 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRZ81_RS26670 (LRZ81_26670) 5395696..5396079 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ81_RS26675 (LRZ81_26675) 5396079..5396321 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRZ81_RS26680 (LRZ81_26680) 5396526..5397443 + 918 WP_002882812.1 alpha/beta hydrolase -
LRZ81_RS26685 (LRZ81_26685) 5397457..5398398 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRZ81_RS26690 (LRZ81_26690) 5398443..5398880 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRZ81_RS26695 (LRZ81_26695) 5398877..5399737 - 861 WP_002882807.1 virulence factor BrkB family protein -
LRZ81_RS26700 (LRZ81_26700) 5399731..5400330 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227278 WP_002882817.1 NZ_CP088991:c5396079-5395696 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227278 NZ_CP122823:1953405-1953659 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227278 WP_004150355.1 NZ_CP088991:c5396321-5396079 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227278 NZ_CP122823:1953157-1953408 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References