Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4198404..4199023 Replicon chromosome
Accession NZ_CP088991
Organism Klebsiella pneumoniae strain 50700

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ81_RS20935 Protein ID WP_002892050.1
Coordinates 4198805..4199023 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ81_RS20930 Protein ID WP_002892066.1
Coordinates 4198404..4198778 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ81_RS20920 (LRZ81_20920) 4193556..4194749 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ81_RS20925 (LRZ81_20925) 4194772..4197918 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ81_RS20930 (LRZ81_20930) 4198404..4198778 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ81_RS20935 (LRZ81_20935) 4198805..4199023 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ81_RS20940 (LRZ81_20940) 4199182..4199748 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ81_RS20945 (LRZ81_20945) 4199720..4199860 - 141 WP_004147370.1 hypothetical protein -
LRZ81_RS20950 (LRZ81_20950) 4199881..4200351 + 471 WP_002892026.1 YlaC family protein -
LRZ81_RS20955 (LRZ81_20955) 4200326..4201777 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LRZ81_RS20960 (LRZ81_20960) 4201878..4202576 + 699 WP_002892021.1 GNAT family protein -
LRZ81_RS20965 (LRZ81_20965) 4202573..4202713 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ81_RS20970 (LRZ81_20970) 4202713..4202976 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227274 WP_002892050.1 NZ_CP088991:4198805-4199023 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227274 NZ_CP122823:761726-762022 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227274 WP_002892066.1 NZ_CP088991:4198404-4198778 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227274 NZ_CP122823:762024-762419 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References