Detailed information of TA system TA227260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 153866..154536
• Replicon: plasmid p53322-228.1
• Accession: NZ_CP088990
• Organism: Klebsiella pneumoniae strain 53322

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LRZ75_RS27365
• Protein ID: WP_004213072.1
• Coordinates: 153866..154309 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRZ75_RS27370
• Protein ID: WP_004213073.1
• Coordinates: 154306..154536 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ75_RS27330 (LRZ75_27330)	149275..149550	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRZ75_RS27335 (LRZ75_27335)	149613..150104	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRZ75_RS27340 (LRZ75_27340)	150153..151073	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
LRZ75_RS27345 (LRZ75_27345)	151164..151567	+	404	Protein_166	GAF domain-containing protein	-
LRZ75_RS27350 (LRZ75_27350)	152086..152722	-	637	Protein_167	mucoid phenotype regulator RmpA2	-
LRZ75_RS27355 (LRZ75_27355)	153139..153443	+	305	Protein_168	transposase	-
LRZ75_RS27360 (LRZ75_27360)	153466..153717	-	252	WP_186987481.1	hypothetical protein	-
LRZ75_RS27365 (LRZ75_27365)	153866..154309	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ75_RS27370 (LRZ75_27370)	154306..154536	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ75_RS27375 (LRZ75_27375)	155144..156277	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRZ75_RS27380 (LRZ75_27380)	156293..156586	+	294	WP_004213076.1	hypothetical protein	-
LRZ75_RS27385 (LRZ75_27385)	156576..156782	-	207	WP_004213077.1	hypothetical protein	-
LRZ75_RS27390 (LRZ75_27390)	157134..157424	+	291	WP_004213078.1	hypothetical protein	-
LRZ75_RS27395 (LRZ75_27395)	157414..158313	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228110	228110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227260 WP_004213072.1 NZ_CP088990:c154309-153866 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227260 NZ_CP122816:3694588-3694806 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227260 WP_004213073.1 NZ_CP088990:c154536-154306 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227260 NZ_CP122816:3694188-3694562 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620