Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 112224..112960 Replicon plasmid p53322-228.1
Accession NZ_CP088990
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LRZ75_RS27125 Protein ID WP_004098919.1
Coordinates 112478..112960 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LRZ75_RS27120 Protein ID WP_004213599.1
Coordinates 112224..112490 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS27095 (LRZ75_27095) 107456..108013 + 558 WP_004213590.1 recombinase family protein -
LRZ75_RS27100 (LRZ75_27100) 108016..110985 + 2970 WP_004213592.1 Tn3 family transposase -
LRZ75_RS27105 (LRZ75_27105) 111027..111521 + 495 WP_004213594.1 hypothetical protein -
LRZ75_RS27110 (LRZ75_27110) 111582..111785 + 204 WP_004213596.1 HHA domain-containing protein -
LRZ75_RS27115 (LRZ75_27115) 111799..112029 + 231 WP_004213598.1 hypothetical protein -
LRZ75_RS27120 (LRZ75_27120) 112224..112490 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LRZ75_RS27125 (LRZ75_27125) 112478..112960 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LRZ75_RS27130 (LRZ75_27130) 113381..114608 + 1228 Protein_123 IS3 family transposase -
LRZ75_RS27135 (LRZ75_27135) 114604..114999 - 396 Protein_124 IS3 family transposase -
LRZ75_RS27140 (LRZ75_27140) 115174..116196 - 1023 WP_004214536.1 porphobilinogen synthase -
LRZ75_RS27145 (LRZ75_27145) 116260..117606 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..228110 228110
- inside IScluster/Tn - - 107456..114608 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T227259 WP_004098919.1 NZ_CP088990:112478-112960 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T227259 NZ_CP122816:2848576-2848683 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT227259 WP_004213599.1 NZ_CP088990:112224-112490 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227259 NZ_CP122816:c2848523-2848462 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References