Detailed information of TA system TA227258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 43611..44521
• Replicon: plasmid p53322-228.1
• Accession: NZ_CP088990
• Organism: Klebsiella pneumoniae strain 53322

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LRZ75_RS26765
• Protein ID: WP_004026354.1
• Coordinates: 44051..44521 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LRZ75_RS26760
• Protein ID: WP_004026357.1
• Coordinates: 43611..44054 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ75_RS26730 (LRZ75_26730)	38629..39150	+	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-
LRZ75_RS26735 (LRZ75_26735)	39147..40100	+	954	Protein_44	fec operon regulator FecR	-
LRZ75_RS26740 (LRZ75_26740)	40187..42313	+	2127	WP_029884227.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
LRZ75_RS26745 (LRZ75_26745)	42388..42513	+	126	Protein_46	Fe3+-citrate ABC transporter substrate-binding protein	-
LRZ75_RS26750 (LRZ75_26750)	42688..42909	+	222	WP_004213643.1	DUF2188 domain-containing protein	-
LRZ75_RS26755 (LRZ75_26755)	43007..43510	+	504	Protein_48	DUF4113 domain-containing protein	-
LRZ75_RS26760 (LRZ75_26760)	43611..44054	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LRZ75_RS26765 (LRZ75_26765)	44051..44521	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LRZ75_RS26770 (LRZ75_26770)	44976..45164	-	189	WP_223271245.1	hypothetical protein	-
LRZ75_RS26775 (LRZ75_26775)	45778..46038	-	261	WP_004026352.1	hypothetical protein	-
LRZ75_RS26780 (LRZ75_26780)	46609..47790	-	1182	WP_004883380.1	IS481 family transposase	-
LRZ75_RS26785 (LRZ75_26785)	47862..48020	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
LRZ75_RS26790 (LRZ75_26790)	48399..48641	-	243	WP_071826037.1	hypothetical protein	-
LRZ75_RS26795 (LRZ75_26795)	48717..48881	-	165	WP_011154586.1	hypothetical protein	-
LRZ75_RS26800 (LRZ75_26800)	48998..49159	-	162	WP_164507110.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228110	228110
	-	flank	IS/Tn	-	-	46609..47790	1181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T227258 WP_004026354.1 NZ_CP088990:44051-44521 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T227258 NZ_CP122816:2848040-2848147 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT227258 WP_004026357.1 NZ_CP088990:43611-44054 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT227258 NZ_CP122816:c2847993-2847927 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9