Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5295663..5296288 Replicon chromosome
Accession NZ_CP088988
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRZ75_RS25385 Protein ID WP_002882817.1
Coordinates 5295663..5296046 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRZ75_RS25390 Protein ID WP_004150355.1
Coordinates 5296046..5296288 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS25370 (5293029) 5293029..5293931 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRZ75_RS25375 (5293928) 5293928..5294563 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRZ75_RS25380 (5294560) 5294560..5295489 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRZ75_RS25385 (5295663) 5295663..5296046 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ75_RS25390 (5296046) 5296046..5296288 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRZ75_RS25395 (5296493) 5296493..5297410 + 918 WP_029884108.1 alpha/beta hydrolase -
LRZ75_RS25400 (5297424) 5297424..5298365 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRZ75_RS25405 (5298410) 5298410..5298847 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRZ75_RS25410 (5298844) 5298844..5299704 - 861 WP_002882807.1 virulence factor BrkB family protein -
LRZ75_RS25415 (5299698) 5299698..5300297 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227255 WP_002882817.1 NZ_CP088988:c5296046-5295663 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227255 NZ_CP122816:2193441-2193544 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227255 WP_004150355.1 NZ_CP088988:c5296288-5296046 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227255 NZ_CP122816:c2193582-2193311 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References