Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4681192..4682002 Replicon chromosome
Accession NZ_CP088988
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LRZ75_RS22525 Protein ID WP_004178461.1
Coordinates 4681192..4681725 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag LRZ75_RS22530 Protein ID WP_004222378.1
Coordinates 4681736..4682002 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS22520 (4680023) 4680023..4681144 + 1122 WP_002887282.1 cupin domain-containing protein -
LRZ75_RS22525 (4681192) 4681192..4681725 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LRZ75_RS22530 (4681736) 4681736..4682002 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LRZ75_RS22535 (4682105) 4682105..4683538 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
LRZ75_RS22540 (4683528) 4683528..4684211 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LRZ75_RS22545 (4684383) 4684383..4685768 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
LRZ75_RS22550 (4685786) 4685786..4686130 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T227253 WP_004178461.1 NZ_CP088988:c4681725-4681192 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T227253 NZ_CP122816:1922099-1922353 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT227253 WP_004222378.1 NZ_CP088988:c4682002-4681736 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT227253 NZ_CP122816:1921851-1922102 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References