Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4077133..4077752 Replicon chromosome
Accession NZ_CP088988
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ75_RS19630 Protein ID WP_002892050.1
Coordinates 4077534..4077752 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ75_RS19625 Protein ID WP_002892066.1
Coordinates 4077133..4077507 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS19615 (4072285) 4072285..4073478 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ75_RS19620 (4073501) 4073501..4076647 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ75_RS19625 (4077133) 4077133..4077507 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ75_RS19630 (4077534) 4077534..4077752 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ75_RS19635 (4077911) 4077911..4078477 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ75_RS19640 (4078449) 4078449..4078589 - 141 WP_004147370.1 hypothetical protein -
LRZ75_RS19645 (4078610) 4078610..4079080 + 471 WP_002892026.1 YlaC family protein -
LRZ75_RS19650 (4079087) 4079087..4080544 - 1458 WP_029883993.1 PLP-dependent aminotransferase family protein -
LRZ75_RS19655 (4080645) 4080645..4081343 + 699 WP_002892021.1 GNAT family protein -
LRZ75_RS19660 (4081340) 4081340..4081480 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ75_RS19665 (4081480) 4081480..4081743 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227252 WP_002892050.1 NZ_CP088988:4077534-4077752 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227252 NZ_CP122816:1921851-1922102 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227252 WP_002892066.1 NZ_CP088988:4077133-4077507 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227252 NZ_CP122816:1922099-1922353 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References