Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 201332..202071 Replicon chromosome
Accession NZ_CP088988
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag LRZ75_RS00985 Protein ID WP_004901283.1
Coordinates 201586..202071 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LRZ75_RS00980 Protein ID WP_003026799.1
Coordinates 201332..201598 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS00965 (196836) 196836..198905 + 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -
LRZ75_RS00970 (199201) 199201..200570 + 1370 WP_087661561.1 IS3 family transposase -
LRZ75_RS00975 (200771) 200771..201199 + 429 WP_004901287.1 GFA family protein -
LRZ75_RS00980 (201332) 201332..201598 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LRZ75_RS00985 (201586) 201586..202071 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
LRZ75_RS00990 (202396) 202396..203442 - 1047 WP_022615678.1 IS481-like element ISKpn28 family transposase -
LRZ75_RS00995 (203516) 203516..203668 + 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
LRZ75_RS01000 (203970) 203970..205589 + 1620 WP_029884695.1 ATP-binding cassette domain-containing protein -
LRZ75_RS01005 (205688) 205688..205900 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
LRZ75_RS01010 (206152) 206152..206442 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 199845..203442 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T227239 WP_004901283.1 NZ_CP088988:201586-202071 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T227239 NZ_CP122807:51157-51432 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT227239 WP_003026799.1 NZ_CP088988:201332-201598 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227239 NZ_CP122807:50873-51157 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGTCTCAGTACTGACAATCCGTTACCGGTGCCCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References