Detailed information of TA system TA227238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 186779..187530
• Replicon: plasmid p53334-210.3
• Accession: NZ_CP088987
• Organism: Klebsiella pneumoniae strain 53334

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LRZ83_RS26080
• Protein ID: WP_048333033.1
• Coordinates: 186779..187261 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: LRZ83_RS26085
• Protein ID: WP_004902250.1
• Coordinates: 187252..187530 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ83_RS26060 (LRZ83_26060)	183178..183825	-	648	WP_014386537.1	EcsC family protein	-
LRZ83_RS26065 (LRZ83_26065)	183852..184607	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
LRZ83_RS26070 (LRZ83_26070)	184708..185100	-	393	WP_032442757.1	hypothetical protein	-
LRZ83_RS26075 (LRZ83_26075)	185205..185744	-	540	WP_004902239.1	hypothetical protein	-
LRZ83_RS26080 (LRZ83_26080)	186779..187261	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
LRZ83_RS26085 (LRZ83_26085)	187252..187530	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
LRZ83_RS26090 (LRZ83_26090)	187652..187864	-	213	WP_016946198.1	hypothetical protein	-
LRZ83_RS26095 (LRZ83_26095)	187972..188313	-	342	WP_032437747.1	hypothetical protein	-
LRZ83_RS26100 (LRZ83_26100)	189219..189533	+	315	WP_023288299.1	hypothetical protein	-
LRZ83_RS26105 (LRZ83_26105)	189777..190259	-	483	WP_172694439.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA	1..210278	210278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T227238 WP_048333033.1 NZ_CP088987:c187261-186779 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T227238 NZ_CP122807:15072-15350 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCAAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT227238 WP_004902250.1 NZ_CP088987:c187530-187252 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT227238 NZ_CP122807:14803-15072 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCGGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3