Detailed information of TA system TA227237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 143302..143972
• Replicon: plasmid p53334-210.3
• Accession: NZ_CP088987
• Organism: Klebsiella pneumoniae strain 53334

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LRZ83_RS25855
• Protein ID: WP_004213072.1
• Coordinates: 143302..143745 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRZ83_RS25860
• Protein ID: WP_004213073.1
• Coordinates: 143742..143972 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ83_RS25820 (LRZ83_25820)	138709..138984	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRZ83_RS25825 (LRZ83_25825)	139047..139538	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRZ83_RS25830 (LRZ83_25830)	139587..140507	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LRZ83_RS25835 (LRZ83_25835)	140598..141001	+	404	Protein_151	GAF domain-containing protein	-
LRZ83_RS25840 (LRZ83_25840)	141518..142152	-	635	Protein_152	mucoid phenotype regulator RmpA2	-
LRZ83_RS25845 (LRZ83_25845)	142575..142879	+	305	Protein_153	transposase	-
LRZ83_RS25850 (LRZ83_25850)	142902..143153	-	252	WP_186987481.1	hypothetical protein	-
LRZ83_RS25855 (LRZ83_25855)	143302..143745	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ83_RS25860 (LRZ83_25860)	143742..143972	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ83_RS25865 (LRZ83_25865)	144580..145713	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRZ83_RS25870 (LRZ83_25870)	145729..146022	+	294	WP_004213076.1	hypothetical protein	-
LRZ83_RS25875 (LRZ83_25875)	146012..146218	-	207	WP_004213077.1	hypothetical protein	-
LRZ83_RS25880 (LRZ83_25880)	146570..146860	+	291	WP_004213078.1	hypothetical protein	-
LRZ83_RS25885 (LRZ83_25885)	146850..147749	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA	1..210278	210278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227237 WP_004213072.1 NZ_CP088987:c143745-143302 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227237 NZ_CP122804:69555-69833 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227237 WP_004213073.1 NZ_CP088987:c143972-143742 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227237 NZ_CP122804:69286-69555 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620