Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 77828..78564 Replicon plasmid p53334-210.3
Accession NZ_CP088987
Organism Klebsiella pneumoniae strain 53334

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LRZ83_RS25520 Protein ID WP_004098919.1
Coordinates 77828..78310 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LRZ83_RS25525 Protein ID WP_004213599.1
Coordinates 78298..78564 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ83_RS25500 (LRZ83_25500) 73182..74528 + 1347 WP_048333011.1 dihydroorotase -
LRZ83_RS25505 (LRZ83_25505) 74592..75614 + 1023 WP_004214536.1 porphobilinogen synthase -
LRZ83_RS25510 (LRZ83_25510) 75789..76184 + 396 Protein_86 IS3 family transposase -
LRZ83_RS25515 (LRZ83_25515) 76180..77407 - 1228 Protein_87 IS3 family transposase -
LRZ83_RS25520 (LRZ83_25520) 77828..78310 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LRZ83_RS25525 (LRZ83_25525) 78298..78564 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LRZ83_RS25530 (LRZ83_25530) 78759..78989 - 231 WP_004213598.1 hypothetical protein -
LRZ83_RS25535 (LRZ83_25535) 79003..79206 - 204 WP_004213596.1 HHA domain-containing protein -
LRZ83_RS25540 (LRZ83_25540) 79267..79761 - 495 WP_004213594.1 hypothetical protein -
LRZ83_RS25545 (LRZ83_25545) 79803..82772 - 2970 WP_004213592.1 Tn3 family transposase -
LRZ83_RS25550 (LRZ83_25550) 82775..83332 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA 1..210278 210278
- inside IScluster/Tn - - 66463..83332 16869


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T227236 WP_004098919.1 NZ_CP088987:c78310-77828 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T227236 NZ_CP122804:53725-53973 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT227236 WP_004213599.1 NZ_CP088987:c78564-78298 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227236 NZ_CP122804:53970-54317 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References