Detailed information of TA system TA227235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 37659..38569
• Replicon: plasmid p53334-210.3
• Accession: NZ_CP088987
• Organism: Klebsiella pneumoniae strain 53334

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LRZ83_RS25305
• Protein ID: WP_004026354.1
• Coordinates: 38099..38569 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LRZ83_RS25300
• Protein ID: WP_004026357.1
• Coordinates: 37659..38102 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ83_RS25270 (LRZ83_25270)	32677..33198	+	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-
LRZ83_RS25275 (LRZ83_25275)	33195..34148	+	954	Protein_38	fec operon regulator FecR	-
LRZ83_RS25280 (LRZ83_25280)	34235..36361	+	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
LRZ83_RS25285 (LRZ83_25285)	36436..36561	+	126	Protein_40	Fe3+-citrate ABC transporter substrate-binding protein	-
LRZ83_RS25290 (LRZ83_25290)	36736..36957	+	222	WP_004213643.1	DUF2188 domain-containing protein	-
LRZ83_RS25295 (LRZ83_25295)	37055..37558	+	504	Protein_42	DUF4113 domain-containing protein	-
LRZ83_RS25300 (LRZ83_25300)	37659..38102	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LRZ83_RS25305 (LRZ83_25305)	38099..38569	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LRZ83_RS25310 (LRZ83_25310)	39024..39212	-	189	WP_223271245.1	hypothetical protein	-
LRZ83_RS25315 (LRZ83_25315)	39386..40083	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LRZ83_RS25320 (LRZ83_25320)	41469..42119	-	651	WP_068893702.1	DUF1173 family protein	-
LRZ83_RS25325 (LRZ83_25325)	42152..42418	-	267	WP_223175074.1	DUF1173 family protein	-
LRZ83_RS25330 (LRZ83_25330)	42596..43090	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA	1..210278	210278
	-	flank	IS/Tn	-	-	39580..40083	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T227235 WP_004026354.1 NZ_CP088987:38099-38569 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T227235 NZ_CP122804:51932-52192 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGC
GAGGGAAAAAATGCTAAATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT227235 WP_004026357.1 NZ_CP088987:37659-38102 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT227235 NZ_CP122804:52182-52433 [Escherichia coli]
ATGCTAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTCCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9