Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4981760..4982385 Replicon chromosome
Accession NZ_CP088985
Organism Klebsiella pneumoniae strain 53334

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRZ83_RS24135 Protein ID WP_002882817.1
Coordinates 4981760..4982143 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRZ83_RS24140 Protein ID WP_004150355.1
Coordinates 4982143..4982385 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ83_RS24120 (LRZ83_24120) 4979126..4980028 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRZ83_RS24125 (LRZ83_24125) 4980025..4980660 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRZ83_RS24130 (LRZ83_24130) 4980657..4981586 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRZ83_RS24135 (LRZ83_24135) 4981760..4982143 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ83_RS24140 (LRZ83_24140) 4982143..4982385 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRZ83_RS24145 (LRZ83_24145) 4982590..4983507 + 918 WP_021466676.1 alpha/beta hydrolase -
LRZ83_RS24150 (LRZ83_24150) 4983521..4984462 - 942 WP_073558103.1 fatty acid biosynthesis protein FabY -
LRZ83_RS24155 (LRZ83_24155) 4984507..4984944 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRZ83_RS24160 (LRZ83_24160) 4984941..4985801 - 861 WP_004146232.1 virulence factor BrkB family protein -
LRZ83_RS24165 (LRZ83_24165) 4985795..4986394 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227234 WP_002882817.1 NZ_CP088985:c4982143-4981760 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227234 NZ_CP122804:34767-34919 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227234 WP_004150355.1 NZ_CP088985:c4982385-4982143 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227234 NZ_CP122804:c34712-34655 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References