Detailed information of TA system TA227233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4662544..4664225
• Replicon: chromosome
• Accession: NZ_CP088985
• Organism: Klebsiella pneumoniae strain 53334

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A2L0KL13
• Locus tag: LRZ83_RS22655
• Protein ID: WP_019704723.1
• Coordinates: 4662544..4663461 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LRZ83_RS22660
• Protein ID: WP_004177809.1
• Coordinates: 4663467..4664225 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ83_RS22635 (LRZ83_22635)	4658116..4659636	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
LRZ83_RS22640 (LRZ83_22640)	4659633..4660631	+	999	WP_002885172.1	ribose ABC transporter permease	-
LRZ83_RS22645 (LRZ83_22645)	4660662..4661603	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LRZ83_RS22650 (LRZ83_22650)	4661678..4662469	+	792	Protein_4441	PfkB family carbohydrate kinase	-
LRZ83_RS22655 (LRZ83_22655)	4662544..4663461	-	918	WP_019704723.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LRZ83_RS22660 (LRZ83_22660)	4663467..4664225	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LRZ83_RS22665 (LRZ83_22665)	4664311..4665039	-	729	WP_002885152.1	response regulator transcription factor	-
LRZ83_RS22670 (LRZ83_22670)	4665312..4667459	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
LRZ83_RS22675 (LRZ83_22675)	4667689..4668375	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34632.07 Da
• Isoelectric Point: 5.4707

>T227233 WP_019704723.1 NZ_CP088985:c4663461-4662544 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T227233 NZ_CP122801:c4803066-4802881 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTCTCTCTTTTAGGAGCTGTAGTTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT227233 WP_004177809.1 NZ_CP088985:c4664225-4663467 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT227233 NZ_CP122801:c4803390-4803094 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A2L0KL13

Antitoxin

• AlphaFold DB: A6TH05