Detailed information of TA system TA227232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4492779..4493295
• Replicon: chromosome
• Accession: NZ_CP088985
• Organism: Klebsiella pneumoniae strain 53334

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LRZ83_RS21795
• Protein ID: WP_040225317.1
• Coordinates: 4492779..4493063 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: LRZ83_RS21800
• Protein ID: WP_002886901.1
• Coordinates: 4493053..4493295 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ83_RS21770 (LRZ83_21770)	4488251..4488514	-	264	WP_025368518.1	PTS sugar transporter subunit IIB	-
LRZ83_RS21775 (LRZ83_21775)	4488644..4488817	+	174	WP_002886906.1	hypothetical protein	-
LRZ83_RS21780 (LRZ83_21780)	4488820..4489563	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LRZ83_RS21785 (LRZ83_21785)	4489920..4492058	+	2139	WP_004222153.1	anaerobic ribonucleoside-triphosphate reductase	-
LRZ83_RS21790 (LRZ83_21790)	4492311..4492775	+	465	WP_004178375.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LRZ83_RS21795 (LRZ83_21795)	4492779..4493063	-	285	WP_040225317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRZ83_RS21800 (LRZ83_21800)	4493053..4493295	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LRZ83_RS21805 (LRZ83_21805)	4493373..4495283	-	1911	WP_009486549.1	PRD domain-containing protein	-
LRZ83_RS21810 (LRZ83_21810)	4495306..4496460	-	1155	WP_002886900.1	lactonase family protein	-
LRZ83_RS21815 (LRZ83_21815)	4496527..4497267	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11172.02 Da
• Isoelectric Point: 10.3787

>T227232 WP_040225317.1 NZ_CP088985:c4493063-4492779 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNLRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T227232 NZ_CP122801:4559031-4559288 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT227232 WP_002886901.1 NZ_CP088985:c4493295-4493053 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT227232 NZ_CP122801:4559300-4559845 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTTAGCAAAGAAGCCGATTTAGTGACCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0