Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4396845..4397655 Replicon chromosome
Accession NZ_CP088985
Organism Klebsiella pneumoniae strain 53334

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LRZ83_RS21340 Protein ID WP_004178461.1
Coordinates 4396845..4397378 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LRZ83_RS21345 Protein ID WP_002887278.1
Coordinates 4397389..4397655 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ83_RS21335 (LRZ83_21335) 4395676..4396797 + 1122 WP_012737335.1 cupin domain-containing protein -
LRZ83_RS21340 (LRZ83_21340) 4396845..4397378 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LRZ83_RS21345 (LRZ83_21345) 4397389..4397655 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LRZ83_RS21350 (LRZ83_21350) 4397758..4399191 - 1434 WP_040183236.1 Cu(+)/Ag(+) sensor histidine kinase -
LRZ83_RS21355 (LRZ83_21355) 4399181..4399864 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
LRZ83_RS21360 (LRZ83_21360) 4400037..4401422 + 1386 WP_073558037.1 efflux transporter outer membrane subunit -
LRZ83_RS21365 (LRZ83_21365) 4401440..4401784 + 345 WP_073558038.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T227231 WP_004178461.1 NZ_CP088985:c4397378-4396845 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T227231 NZ_CP122801:4151270-4151548 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT227231 WP_002887278.1 NZ_CP088985:c4397655-4397389 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT227231 NZ_CP122801:4151007-4151267 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References