Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3790443..3791062 Replicon chromosome
Accession NZ_CP088985
Organism Klebsiella pneumoniae strain 53334

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ83_RS18405 Protein ID WP_002892050.1
Coordinates 3790844..3791062 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ83_RS18400 Protein ID WP_002892066.1
Coordinates 3790443..3790817 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ83_RS18390 (LRZ83_18390) 3785595..3786788 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ83_RS18395 (LRZ83_18395) 3786811..3789957 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ83_RS18400 (LRZ83_18400) 3790443..3790817 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ83_RS18405 (LRZ83_18405) 3790844..3791062 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ83_RS18410 (LRZ83_18410) 3791221..3791787 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ83_RS18415 (LRZ83_18415) 3791759..3791899 - 141 WP_004147370.1 hypothetical protein -
LRZ83_RS18420 (LRZ83_18420) 3791920..3792390 + 471 WP_002892026.1 YlaC family protein -
LRZ83_RS18425 (LRZ83_18425) 3792365..3793816 - 1452 WP_073557971.1 PLP-dependent aminotransferase family protein -
LRZ83_RS18430 (LRZ83_18430) 3793917..3794615 + 699 WP_002892021.1 GNAT family protein -
LRZ83_RS18435 (LRZ83_18435) 3794612..3794752 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ83_RS18440 (LRZ83_18440) 3794752..3795015 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227230 WP_002892050.1 NZ_CP088985:3790844-3791062 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227230 NZ_CP122801:c4145208-4144810 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227230 WP_002892066.1 NZ_CP088985:3790443-3790817 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227230 NZ_CP122801:c4145504-4145211 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References