Detailed information of TA system TA227215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 102317..103227
• Replicon: plasmid p53357-227.9
• Accession: NZ_CP088984
• Organism: Klebsiella pneumoniae strain 53357

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LRZ78_RS27440
• Protein ID: WP_004026354.1
• Coordinates: 102317..102787 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LRZ78_RS27445
• Protein ID: WP_004026357.1
• Coordinates: 102784..103227 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ78_RS27415 (LRZ78_27420)	97729..98127	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
LRZ78_RS27420 (LRZ78_27425)	98464..98958	-	495	WP_004212794.1	thermonuclease family protein	-
LRZ78_RS27425 (LRZ78_27430)	99136..99402	+	267	WP_223175074.1	DUF1173 family protein	-
LRZ78_RS27430 (LRZ78_27435)	99435..100085	+	651	WP_068893702.1	DUF1173 family protein	-
LRZ78_RS27440 (LRZ78_27445)	102317..102787	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LRZ78_RS27445 (LRZ78_27450)	102784..103227	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LRZ78_RS27450 (LRZ78_27455)	103328..103831	-	504	Protein_112	DUF4113 domain-containing protein	-
LRZ78_RS27455 (LRZ78_27460)	103929..104150	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
LRZ78_RS27460 (LRZ78_27465)	104325..104450	-	126	Protein_114	Fe3+-citrate ABC transporter substrate-binding protein	-
LRZ78_RS27465 (LRZ78_27470)	104525..106651	-	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
LRZ78_RS27470 (LRZ78_27475)	106738..107691	-	954	Protein_116	fec operon regulator FecR	-
LRZ78_RS27475 (LRZ78_27480)	107688..108209	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..227891	227891
	-	flank	IS/Tn	-	-	101471..101974	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T227215 WP_004026354.1 NZ_CP088984:c102787-102317 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T227215 NZ_CP122772:27089-27364 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT227215 WP_004026357.1 NZ_CP088984:c103227-102784 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT227215 NZ_CP122772:26805-27089 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGTCTCAGTACTGACAATCCGTTACCGGTGCCCCGCTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9