Detailed information of TA system TA022721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 868411..869116
• Replicon: chromosome
• Accession: NC_010498
• Organism: Escherichia coli SMS-3-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: ECSMS35_RS04290
• Protein ID: WP_000539521.1
• Coordinates: 868730..869116 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ECSMS35_RS04285
• Protein ID: WP_001280945.1
• Coordinates: 868411..868740 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSMS35_RS04270	863569..864480	+	912	WP_001236058.1	glutathione ABC transporter permease GsiD	-
ECSMS35_RS04275	864657..867005	+	2349	WP_000950337.1	EAL domain-containing protein	-
ECSMS35_RS04280	867013..868341	+	1329	WP_000086869.1	GGDEF domain-containing protein	-
ECSMS35_RS04285	868411..868740	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
ECSMS35_RS04290	868730..869116	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECSMS35_RS04295	869342..870667	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
ECSMS35_RS04300	870880..871263	+	384	WP_000497137.1	biofilm formation regulator BssR	-
ECSMS35_RS04305	871374..872489	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
ECSMS35_RS04310	872486..873112	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T22721 WP_000539521.1 NC_010498:c869116-868730 [Escherichia coli SMS-3-5]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T22721 NC_010498:c869116-868730 [Escherichia coli SMS-3-5]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT22721 WP_001280945.1 NC_010498:c868740-868411 [Escherichia coli SMS-3-5]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT22721 NC_010498:c868740-868411 [Escherichia coli SMS-3-5]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6