Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4172270..4172889 Replicon chromosome
Accession NZ_CP088982
Organism Klebsiella pneumoniae strain 53357

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ78_RS20120 Protein ID WP_002892050.1
Coordinates 4172671..4172889 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ78_RS20115 Protein ID WP_002892066.1
Coordinates 4172270..4172644 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ78_RS20105 (4167422) 4167422..4168615 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ78_RS20110 (4168638) 4168638..4171784 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ78_RS20115 (4172270) 4172270..4172644 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ78_RS20120 (4172671) 4172671..4172889 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ78_RS20125 (4173048) 4173048..4173614 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ78_RS20130 (4173586) 4173586..4173726 - 141 WP_004147370.1 hypothetical protein -
LRZ78_RS20135 (4173747) 4173747..4174217 + 471 WP_002892026.1 YlaC family protein -
LRZ78_RS20140 (4174192) 4174192..4175643 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
LRZ78_RS20145 (4175744) 4175744..4176442 + 699 WP_002892021.1 GNAT family protein -
LRZ78_RS20150 (4176439) 4176439..4176579 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ78_RS20155 (4176579) 4176579..4176842 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227208 WP_002892050.1 NZ_CP088982:4172671-4172889 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227208 NZ_CP122768:4854988-4855329 [Escherichia coli]
ATGACTGACACGAATTTTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCCCCCGCCGCTGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227208 WP_002892066.1 NZ_CP088982:4172270-4172644 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227208 NZ_CP122768:c4854993-4854917 [Escherichia coli]
AGTCATAACTGCTATTCTCCTGGAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References