Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 508403..509021 Replicon chromosome
Accession NC_010498
Organism Escherichia coli SMS-3-5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECSMS35_RS02500 Protein ID WP_001291435.1
Coordinates 508403..508621 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECSMS35_RS02505 Protein ID WP_000344800.1
Coordinates 508647..509021 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSMS35_RS02470 503693..504265 + 573 WP_000779831.1 YbaY family lipoprotein -
ECSMS35_RS02475 504296..504607 - 312 WP_000409911.1 MGMT family protein -
ECSMS35_RS02480 504986..505339 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECSMS35_RS02485 505380..506930 - 1551 WP_012311818.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECSMS35_RS02490 507094..507564 - 471 WP_000136192.1 YlaC family protein -
ECSMS35_RS02495 507680..508231 - 552 WP_000102561.1 maltose O-acetyltransferase -
ECSMS35_RS02500 508403..508621 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECSMS35_RS02505 508647..509021 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECSMS35_RS02510 509567..512716 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECSMS35_RS02515 512739..513932 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T22720 WP_001291435.1 NC_010498:c508621-508403 [Escherichia coli SMS-3-5]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T22720 NC_010498:c508621-508403 [Escherichia coli SMS-3-5]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT22720 WP_000344800.1 NC_010498:c509021-508647 [Escherichia coli SMS-3-5]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT22720 NC_010498:c509021-508647 [Escherichia coli SMS-3-5]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTCCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References