Detailed information of TA system TA227196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 327689..328275
• Replicon: chromosome
• Accession: NZ_CP088982
• Organism: Klebsiella pneumoniae strain 53357

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A330WAT7
• Locus tag: LRZ78_RS01525
• Protein ID: WP_014906830.1
• Coordinates: 327907..328275 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LRZ78_RS01520
• Protein ID: WP_004174006.1
• Coordinates: 327689..327910 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ78_RS01500 (323846)	323846..324772	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LRZ78_RS01505 (324769)	324769..326046	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LRZ78_RS01510 (326043)	326043..326810	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LRZ78_RS01515 (326812)	326812..327525	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LRZ78_RS01520 (327689)	327689..327910	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LRZ78_RS01525 (327907)	327907..328275	+	369	WP_014906830.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LRZ78_RS01530 (328548)	328548..329864	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LRZ78_RS01535 (329971)	329971..330858	+	888	WP_014906831.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LRZ78_RS01540 (330855)	330855..331700	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LRZ78_RS01545 (331702)	331702..332772	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	322675..333509	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13537.95 Da
• Isoelectric Point: 8.6410

>T227196 WP_014906830.1 NZ_CP088982:327907-328275 [Klebsiella pneumoniae]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T227196 NZ_CP122768:989387-989794 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT227196 WP_004174006.1 NZ_CP088982:327689-327910 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT227196 NZ_CP122768:989140-989406 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A330WAT7

Antitoxin

• AlphaFold DB: A0A5E5YJY7