Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5230962..5231587 Replicon chromosome
Accession NZ_CP088978
Organism Klebsiella pneumoniae strain KP015

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LST75_RS25465 Protein ID WP_002882817.1
Coordinates 5230962..5231345 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LST75_RS25470 Protein ID WP_004150355.1
Coordinates 5231345..5231587 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LST75_RS25450 (LST75_25400) 5228328..5229230 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LST75_RS25455 (LST75_25405) 5229227..5229862 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LST75_RS25460 (LST75_25410) 5229859..5230788 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LST75_RS25465 (LST75_25415) 5230962..5231345 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LST75_RS25470 (LST75_25420) 5231345..5231587 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LST75_RS25475 (LST75_25425) 5231792..5232709 + 918 WP_021466676.1 alpha/beta hydrolase -
LST75_RS25480 (LST75_25430) 5232723..5233664 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LST75_RS25485 (LST75_25435) 5233709..5234146 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LST75_RS25490 (LST75_25440) 5234143..5235003 - 861 WP_004146232.1 virulence factor BrkB family protein -
LST75_RS25495 (LST75_25445) 5234997..5235596 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227190 WP_002882817.1 NZ_CP088978:c5231345-5230962 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227190 NZ_CP122768:274242-274349 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227190 WP_004150355.1 NZ_CP088978:c5231587-5231345 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227190 NZ_CP122768:c274193-274130 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References