Detailed information of TA system TA022719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 474095..474932
• Replicon: chromosome
• Accession: NC_010498
• Organism: Escherichia coli SMS-3-5

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: ECSMS35_RS02330
• Protein ID: WP_000227787.1
• Coordinates: 474095..474637 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: ECSMS35_RS02335
• Protein ID: WP_001353405.1
• Coordinates: 474621..474932 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSMS35_RS02310	469635..470546	-	912	WP_000705871.1	2-dehydropantoate 2-reductase	-
ECSMS35_RS02315	470714..471205	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECSMS35_RS02320	471333..472697	-	1365	WP_001000978.1	MFS transporter	-
ECSMS35_RS02325	473104..474039	+	936	WP_012311701.1	sel1 repeat family protein	-
ECSMS35_RS02330	474095..474637	-	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
ECSMS35_RS02335	474621..474932	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
ECSMS35_RS02340	475117..476007	-	891	WP_000971336.1	heme o synthase	-
ECSMS35_RS02345	476019..476348	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECSMS35_RS02350	476348..476962	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECSMS35_RS02355	476952..478943	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECSMS35_RS02360	478965..479912	-	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T22719 WP_000227787.1 NC_010498:c474637-474095 [Escherichia coli SMS-3-5]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T22719 NC_010498:c474637-474095 [Escherichia coli SMS-3-5]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT22719 WP_001353405.1 NC_010498:c474932-474621 [Escherichia coli SMS-3-5]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT22719 NC_010498:c474932-474621 [Escherichia coli SMS-3-5]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGCCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3