Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4019220..4019839 Replicon chromosome
Accession NZ_CP088978
Organism Klebsiella pneumoniae strain KP015

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LST75_RS19700 Protein ID WP_002892050.1
Coordinates 4019621..4019839 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LST75_RS19695 Protein ID WP_002892066.1
Coordinates 4019220..4019594 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LST75_RS19685 (LST75_19645) 4014372..4015565 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LST75_RS19690 (LST75_19650) 4015588..4018734 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LST75_RS19695 (LST75_19655) 4019220..4019594 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LST75_RS19700 (LST75_19660) 4019621..4019839 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LST75_RS19705 (LST75_19665) 4019998..4020564 + 567 WP_002892030.1 maltose O-acetyltransferase -
LST75_RS19710 (LST75_19670) 4020536..4020676 - 141 WP_004147370.1 hypothetical protein -
LST75_RS19715 (LST75_19675) 4020697..4021167 + 471 WP_040187972.1 YlaC family protein -
LST75_RS19720 (LST75_19680) 4021142..4022593 - 1452 WP_040187973.1 PLP-dependent aminotransferase family protein -
LST75_RS19725 (LST75_19685) 4022694..4023392 + 699 WP_002892021.1 GNAT family protein -
LST75_RS19730 (LST75_19690) 4023389..4023529 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LST75_RS19735 (LST75_19695) 4023529..4023792 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227187 WP_002892050.1 NZ_CP088978:4019621-4019839 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227187 NZ_CP122766:c43970-43695 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227187 WP_002892066.1 NZ_CP088978:4019220-4019594 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227187 NZ_CP122766:c44254-43970 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References