Detailed information of TA system TA022718

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 317865..318571
• Replicon: chromosome
• Accession: NC_010498
• Organism: Escherichia coli SMS-3-5

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: V0SXW1
• Locus tag: ECSMS35_RS01550
• Protein ID: WP_000854677.1
• Coordinates: 318203..318571 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: V0SU84
• Locus tag: ECSMS35_RS01545
• Protein ID: WP_000065824.1
• Coordinates: 317865..318182 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSMS35_RS01520	314956..316133	+	1178	WP_152895938.1	IS3 family transposase	-
ECSMS35_RS01525	316344..316580	+	237	Protein_293	DUF905 domain-containing protein	-
ECSMS35_RS01530	316666..317124	+	459	WP_000211834.1	antirestriction protein	-
ECSMS35_RS01535	317136..317615	+	480	WP_000438147.1	RadC family protein	-
ECSMS35_RS01540	317624..317845	+	222	WP_001220307.1	DUF987 domain-containing protein	-
ECSMS35_RS01545	317865..318182	+	318	WP_000065824.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECSMS35_RS01550	318203..318571	+	369	WP_000854677.1	TA system toxin CbtA family protein	Toxin
ECSMS35_RS01570	319364..319978	+	615	WP_001019920.1	YagU family protein	-
ECSMS35_RS01575	320227..320556	-	330	WP_001303809.1	YkgJ family cysteine cluster protein	-
ECSMS35_RS01580	320869..321579	-	711	WP_001361803.1	fimbrial chaperone EcpE	-
ECSMS35_RS01585	321548..323191	-	1644	WP_001265639.1	fimbrial adhesin EcpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	308165..346902	38737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.89 Da
• Isoelectric Point: 8.2671

>T22718 WP_000854677.1 NC_010498:318203-318571 [Escherichia coli SMS-3-5]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T22718 NC_010498:318203-318571 [Escherichia coli SMS-3-5]
ATGAAAACTCTACCTGCAACGACTCCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTTAC
TCGTCTACTTGAGCAGCACTATGGCCTAATGTTAAGCGACACACCTTTTAGCGATGAAACAGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTTGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTACGACAGACATTCTTCAAGCTAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11964.46 Da
• Isoelectric Point: 6.3163

>AT22718 WP_000065824.1 NC_010498:317865-318182 [Escherichia coli SMS-3-5]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT22718 NC_010498:317865-318182 [Escherichia coli SMS-3-5]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACACCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAACT
GCATTATTTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTTACCACTGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
GATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTACTCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: V0SXW1

Antitoxin

• AlphaFold DB: V0SU84