Detailed information of TA system TA227173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 472665..473164
• Replicon: chromosome
• Accession: NZ_CP088975
• Organism: Rhodococcus pyridinivorans strain DNHP-S2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H0JMW2
• Locus tag: LSF60_RS02215
• Protein ID: WP_006550854.1
• Coordinates: 472910..473164 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H0JMW3
• Locus tag: LSF60_RS02210
• Protein ID: WP_006550855.1
• Coordinates: 472665..472913 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSF60_RS02195 (LSF60_02195)	469728..470978	-	1251	WP_138845195.1	alpha/beta fold hydrolase	-
LSF60_RS02200 (LSF60_02200)	471039..471833	-	795	WP_006550857.1	LLM class F420-dependent oxidoreductase	-
LSF60_RS02205 (LSF60_02205)	472300..472440	-	141	Protein_428	hypothetical protein	-
LSF60_RS02210 (LSF60_02210)	472665..472913	+	249	WP_006550855.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LSF60_RS02215 (LSF60_02215)	472910..473164	+	255	WP_006550854.1	Txe/YoeB family addiction module toxin	Toxin
LSF60_RS02220 (LSF60_02220)	473266..473787	-	522	WP_231781374.1	hypothetical protein	-
LSF60_RS02225 (LSF60_02225)	473944..474900	+	957	WP_019290518.1	ERCC4 domain-containing protein	-
LSF60_RS02230 (LSF60_02230)	475463..475675	+	213	WP_052040167.1	hypothetical protein	-
LSF60_RS02235 (LSF60_02235)	476046..477251	-	1206	WP_064851798.1	IS4 family transposase	-
LSF60_RS02240 (LSF60_02240)	477295..477921	-	627	WP_006550849.1	TetR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10064.34 Da
• Isoelectric Point: 6.7171

>T227173 WP_006550854.1 NZ_CP088975:472910-473164 [Rhodococcus pyridinivorans]
VNITFTDEAWDDYQFWISTDRKQLERVNELVRDCQRDPYSGIGKPERLRHALSGLWSRRIDKEHRLVYEVIDGALVIIAA
RYHY

Nucleotide

• Length: 255 bp

>T227173 NZ_CP122758:2346703-2346990 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.59 Da
• Isoelectric Point: 5.8315

>AT227173 WP_006550855.1 NZ_CP088975:472665-472913 [Rhodococcus pyridinivorans]
MAAITATEARKNFFPLLEQVNKDHTPVEIVSKRGNAVLMSKDDYDAMLETSYLMQSPANARRLLASMQRARGGEYTEREL
IE

Nucleotide

• Length: 249 bp

>AT227173 NZ_CP122758:2346464-2346703 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: H0JMW2

Antitoxin

• AlphaFold DB: H0JMW3