Detailed information of TA system TA227171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1784665..1785212
• Replicon: chromosome
• Accession: NZ_CP088965
• Organism: Brucella anthropi strain T210003

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: LRL11_RS22405
• Protein ID: WP_151610180.1
• Coordinates: 1784665..1784910 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LRL11_RS22410
• Protein ID: WP_151610179.1
• Coordinates: 1784910..1785212 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRL11_RS22380 (LRL11_22380)	1781419..1782597	-	1179	WP_151610184.1	right-handed parallel beta-helix repeat-containing protein	-
LRL11_RS22385 (LRL11_22385)	1782738..1783010	-	273	WP_151610183.1	hypothetical protein	-
LRL11_RS22390 (LRL11_22390)	1783011..1783223	-	213	WP_151610182.1	hypothetical protein	-
LRL11_RS22395 (LRL11_22395)	1783436..1784104	-	669	WP_151610181.1	phage terminase large subunit	-
LRL11_RS22400 (LRL11_22400)	1784104..1784589	-	486	WP_192799844.1	hypothetical protein	-
LRL11_RS22405 (LRL11_22405)	1784665..1784910	+	246	WP_151610180.1	BrnT family toxin	Toxin
LRL11_RS22410 (LRL11_22410)	1784910..1785212	+	303	WP_151610179.1	BrnA antitoxin family protein	Antitoxin
LRL11_RS22415 (LRL11_22415)	1785242..1785748	-	507	WP_151610178.1	hypothetical protein	-
LRL11_RS22420 (LRL11_22420)	1785718..1786626	-	909	WP_151610177.1	hypothetical protein	-
LRL11_RS22425 (LRL11_22425)	1786834..1787373	-	540	WP_151610176.1	hypothetical protein	-
LRL11_RS22430 (LRL11_22430)	1787383..1787661	-	279	WP_151610175.1	hypothetical protein	-
LRL11_RS22435 (LRL11_22435)	1787879..1788406	-	528	WP_151610174.1	hypothetical protein	-
LRL11_RS22440 (LRL11_22440)	1788366..1788695	-	330	WP_151610173.1	hypothetical protein	-
LRL11_RS22445 (LRL11_22445)	1788733..1788927	+	195	WP_231771526.1	hypothetical protein	-
LRL11_RS22450 (LRL11_22450)	1788927..1789352	+	426	WP_151610171.1	HNH endonuclease signature motif containing protein	-
LRL11_RS22455 (LRL11_22455)	1789561..1790055	+	495	WP_151610170.1	hypothetical protein	-
LRL11_RS22460 (LRL11_22460)	1790052..1790195	+	144	WP_192799843.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (25-98)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9016.58 Da
• Isoelectric Point: 10.1808

>T227171 WP_151610180.1 NZ_CP088965:1784665-1784910 [Brucella anthropi]
MKIVWDEPKREANLVKHGMDFASLTIEFFEASTIIPAKRGRLQAIGRLADGTIVVIFVTLGTEALSVISMRSARKDERSL
I

Nucleotide

• Length: 246 bp

>T227171 NZ_CP122758:1809335-1809589 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11390.03 Da
• Isoelectric Point: 10.4275

>AT227171 WP_151610179.1 NZ_CP088965:1784910-1785212 [Brucella anthropi]
MANKFNPKHAAEHGYTKEDWDAVSDNPPITKEEMKRAKPFKEALPELYESIQRSRGRPRVASPKEAVTLRLDPDVIQKFK
ATGKNWRGNMTEALRKAVGL

Nucleotide

• Length: 303 bp

>AT227171 NZ_CP122758:1809087-1809338 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA