Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 108741..109384 Replicon plasmid pSMS35_130
Accession NC_010488
Organism Escherichia coli SMS-3-5

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag ECSMS35_RS25615 Protein ID WP_001044768.1
Coordinates 108968..109384 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag ECSMS35_RS25610 Protein ID WP_001261287.1
Coordinates 108741..108971 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSMS35_RS25575 104134..104742 + 609 WP_000050382.1 HAD-IB family hydrolase -
ECSMS35_RS25580 104800..105591 + 792 WP_001261740.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA2 -
ECSMS35_RS25585 105733..106437 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
ECSMS35_RS27900 106500..107216 + 717 Protein_127 exclusion suppressor FxsA -
ECSMS35_RS25600 107266..108165 - 900 WP_000963206.1 nucleotide-binding protein -
ECSMS35_RS25605 108155..108445 - 291 WP_000111771.1 hypothetical protein -
ECSMS35_RS25610 108741..108971 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECSMS35_RS25615 108968..109384 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
ECSMS35_RS25620 109546..111684 - 2139 WP_000350637.1 AAA family ATPase -
ECSMS35_RS25625 112038..112295 + 258 WP_000343085.1 hypothetical protein -
ECSMS35_RS25630 112295..112885 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / catA2 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aadA2 / tet(A) / aph(3')-Ia - 1..130440 130440


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T22717 WP_001044768.1 NC_010488:108968-109384 [Escherichia coli SMS-3-5]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T22717 NC_010488:108968-109384 [Escherichia coli SMS-3-5]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT22717 WP_001261287.1 NC_010488:108741-108971 [Escherichia coli SMS-3-5]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT22717 NC_010488:108741-108971 [Escherichia coli SMS-3-5]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References