Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1075354..1075978 Replicon chromosome
Accession NZ_CP088952
Organism Massilia sp. DM-R-R2A-13

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag LSQ66_RS04940 Protein ID WP_231768686.1
Coordinates 1075796..1075978 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LSQ66_RS04935 Protein ID WP_231770051.1
Coordinates 1075354..1075770 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSQ66_RS04910 (LSQ66_04910) 1070787..1071416 + 630 WP_231768683.1 response regulator transcription factor -
LSQ66_RS04915 (LSQ66_04915) 1071661..1073682 + 2022 WP_231768684.1 EAL domain-containing protein -
LSQ66_RS04925 (LSQ66_04925) 1073901..1074677 + 777 WP_231768685.1 hypothetical protein -
LSQ66_RS04930 (LSQ66_04930) 1074641..1075315 - 675 WP_231770050.1 SOS response-associated peptidase -
LSQ66_RS04935 (LSQ66_04935) 1075354..1075770 - 417 WP_231770051.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LSQ66_RS04940 (LSQ66_04940) 1075796..1075978 - 183 WP_231768686.1 type II toxin-antitoxin system HicA family toxin Toxin
LSQ66_RS04945 (LSQ66_04945) 1076150..1076635 + 486 WP_231768687.1 hypothetical protein -
LSQ66_RS04950 (LSQ66_04950) 1076783..1077511 + 729 WP_231768688.1 DNA-binding protein -
LSQ66_RS04955 (LSQ66_04955) 1077803..1078012 + 210 WP_231768689.1 cold-shock protein -
LSQ66_RS04960 (LSQ66_04960) 1078101..1079312 - 1212 WP_231768690.1 PepSY-associated TM helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1074641..1084645 10004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-126)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.96 Da        Isoelectric Point: 11.2341

>T227162 WP_231768686.1 NZ_CP088952:c1075978-1075796 [Massilia sp. DM-R-R2A-13]
MDSAKLIQILLDAGWRQVRQRGSHVTLKHPLTHKLCTVPHPKKDLPTGTVKSILKNAGLK

Download         Length: 183 bp

>T227162 NZ_CP122732:64437-64712 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTTCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15037.14 Da        Isoelectric Point: 4.3478

>AT227162 WP_231770051.1 NZ_CP088952:c1075770-1075354 [Massilia sp. DM-R-R2A-13]
MELPVVIHKDDASVYGVTVPDIEGCYSWGESIEAALRNTKDAVQSHVEAMIDEGIAVNIVPSRIDDLIQMEELAGGIWAL
IDLDPMTLDSKPERINVSIPRFVLAKIDQYASARSETRSGFLARAALQLIRTEKAGAG

Download         Length: 417 bp

>AT227162 NZ_CP122732:64153-64437 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGATCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGACCTGG
GCAGACAGCCTCAATACTGACAATCCGTTACCGGTGCCTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References