Detailed information of TA system TA227161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 3911824..3912482
• Replicon: chromosome
• Accession: NZ_CP088935
• Organism: Thauera sp. WB-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LQF09_RS17870
• Protein ID: WP_270068596.1
• Coordinates: 3912069..3912482 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3F3GB95
• Locus tag: LQF09_RS17865
• Protein ID: WP_002928853.1
• Coordinates: 3911824..3912072 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF09_RS17830 (LQF09_17840)	3907381..3908070	+	690	WP_002928872.1	urea ABC transporter ATP-binding subunit UrtE	-
LQF09_RS17835 (LQF09_17845)	3908092..3909324	+	1233	WP_270068592.1	acetamidase/formamidase family protein	-
LQF09_RS17840 (LQF09_17850)	3909408..3909752	+	345	WP_270068593.1	zinc ribbon domain-containing protein	-
LQF09_RS17845 (LQF09_17855)	3909845..3910096	+	252	WP_270068594.1	ribbon-helix-helix domain-containing protein	-
LQF09_RS17850 (LQF09_17860)	3910093..3910425	+	333	WP_270068595.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LQF09_RS17855 (LQF09_17865)	3910757..3911071	+	315	WP_002925880.1	hypothetical protein	-
LQF09_RS17860 (LQF09_17870)	3911061..3911342	+	282	WP_002925881.1	helix-turn-helix domain-containing protein	-
LQF09_RS17865 (LQF09_17875)	3911824..3912072	+	249	WP_002928853.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LQF09_RS17870 (LQF09_17880)	3912069..3912482	+	414	WP_270068596.1	type II toxin-antitoxin system VapC family toxin	Toxin
LQF09_RS17875 (LQF09_17885)	3912613..3913650	+	1038	WP_270068597.1	aliphatic amidase	-
LQF09_RS17880 (LQF09_17890)	3913735..3914532	+	798	WP_270068598.1	CbbQ/NirQ/NorQ/GpvN family protein	-
LQF09_RS17885 (LQF09_17895)	3914516..3916186	+	1671	WP_270068599.1	VWA domain-containing protein	-
LQF09_RS17890 (LQF09_17900)	3916310..3917062	+	753	WP_270068600.1	flagellar brake protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3904424..3914532	10108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-62)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15106.32 Da
• Isoelectric Point: 5.1663

>T227161 WP_270068596.1 NZ_CP088935:3912069-3912482 [Thauera sp. WB-2]
MSYLIDTNVLSELRRKSPDPGVVAWFSQRPPTTLHLSVLTLGEIRKGIEGVGDEVRKQTLIDWLETDLPTFFTGRILDVN
GAVADRWGRLVAAAGRPLPTIDSLLAATALEHDLVLVTRNTKDFAGLPVEVFNPWTS

Nucleotide

• Length: 414 bp

>T227161 NZ_CP122732:c33425-33027 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9471.73 Da
• Isoelectric Point: 5.8152

>AT227161 WP_002928853.1 NZ_CP088935:3911824-3912072 [Thauera sp. WB-2]
MQTWQMQEAKARMSELVKRAQTQPQDITLHGKSVAVVISRETFDRLSQAQDSLVDFMRRSPLFGTDDIALEREQSLTREV
SF

Nucleotide

• Length: 249 bp

>AT227161 NZ_CP122732:c33652-33425 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3F3GB95