Detailed information of TA system TA227158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/StbC(antitoxin)
• Location: 2827764..2828179
• Replicon: chromosome
• Accession: NZ_CP088935
• Organism: Thauera sp. WB-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LQF09_RS12980
• Protein ID: WP_270068151.1
• Coordinates: 2827764..2827967 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3F3GDF9
• Locus tag: LQF09_RS12985
• Protein ID: WP_002926672.1
• Coordinates: 2827967..2828179 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF09_RS12955 (LQF09_12960)	2823434..2823823	+	390	WP_002926660.1	ectoine synthase	-
LQF09_RS12960 (LQF09_12965)	2823840..2824832	+	993	WP_270068148.1	ectoine hydroxylase	-
LQF09_RS12965 (LQF09_12970)	2824969..2825373	-	405	WP_002926664.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
LQF09_RS12970 (LQF09_12975)	2825370..2825627	-	258	WP_270068149.1	type I restriction endonuclease subunit R	-
LQF09_RS12975 (LQF09_12980)	2826409..2827767	-	1359	WP_270068150.1	IS1182 family transposase	-
LQF09_RS12980 (LQF09_12985)	2827764..2827967	-	204	WP_270068151.1	hypothetical protein	Toxin
LQF09_RS12985 (LQF09_12990)	2827967..2828179	-	213	WP_002926672.1	hypothetical protein	Antitoxin
LQF09_RS12995 (LQF09_13000)	2828608..2829729	-	1122	WP_270068152.1	L-threonylcarbamoyladenylate synthase	-
LQF09_RS13000 (LQF09_13005)	2829740..2830894	-	1155	WP_270068153.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
LQF09_RS13005 (LQF09_13010)	2830905..2831396	-	492	WP_270068154.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
LQF09_RS13010 (LQF09_13015)	2831407..2832273	-	867	WP_270068155.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7401.74 Da
• Isoelectric Point: 10.4283

>T227158 WP_270068151.1 NZ_CP088935:c2827967-2827764 [Thauera sp. WB-2]
MYLLDTNIISELRKGGRANPGVTAFFATLAAEDIYLSVLTIGEIRRGLAACRTTPCKMPHRPRILRA

Nucleotide

• Length: 204 bp

>T227158 NZ_CP122727:c3796976-3796578 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7666.68 Da
• Isoelectric Point: 9.0188

>AT227158 WP_002926672.1 NZ_CP088935:c2828179-2827967 [Thauera sp. WB-2]
MATNLVVRNIEPSVAQALKEAAARHGRSAEAEHREILRAALARPARRTFKDVLASMPDVGADEDFNLRQG

Nucleotide

• Length: 213 bp

>AT227158 NZ_CP122727:c3797272-3796979 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3F3GDF9