Detailed information of TA system TA227156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 692252..692892
• Replicon: chromosome
• Accession: NZ_CP088935
• Organism: Thauera sp. WB-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LQF09_RS03120
• Protein ID: WP_270069915.1
• Coordinates: 692252..692650 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LQF09_RS03125
• Protein ID: WP_270069186.1
• Coordinates: 692653..692892 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF09_RS03095 (LQF09_03095)	687590..688762	-	1173	WP_270069181.1	glycosyltransferase family 4 protein	-
LQF09_RS03100 (LQF09_03100)	688771..689364	-	594	WP_270069182.1	Uma2 family endonuclease	-
LQF09_RS03105 (LQF09_03105)	689422..690324	-	903	WP_270069183.1	mitochondrial fission ELM1 family protein	-
LQF09_RS03110 (LQF09_03110)	690339..691508	-	1170	WP_270069184.1	ATP-binding protein	-
LQF09_RS03115 (LQF09_03115)	691602..692195	-	594	WP_270069185.1	alpha-ribazole phosphatase family protein	-
LQF09_RS03120 (LQF09_03120)	692252..692650	-	399	WP_270069915.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LQF09_RS03125 (LQF09_03125)	692653..692892	-	240	WP_270069186.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LQF09_RS03130 (LQF09_03130)	692941..693708	-	768	WP_270069187.1	adenosylcobinamide-GDP ribazoletransferase	-
LQF09_RS03135 (LQF09_03135)	693715..694761	-	1047	WP_270069188.1	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
LQF09_RS03140 (LQF09_03140)	694817..695365	-	549	WP_270069189.1	bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase	-
LQF09_RS03145 (LQF09_03145)	695832..697661	+	1830	WP_270069190.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14653.77 Da
• Isoelectric Point: 7.3590

>T227156 WP_270069915.1 NZ_CP088935:c692650-692252 [Thauera sp. WB-2]
MLKYLLDTNIVIYTIKNKPAQVRERFRQHDGQMAISSVSLGELVFGAERSAQPEANLAVVEGFVARVESLDFDAAAAHHF
GQIRAELCRAGRPIGPYDMMIAAHARSRGLILVTNNTSEFERVAGLRLENWA

Nucleotide

• Length: 399 bp

>T227156 NZ_CP122727:c2852215-2851838 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8777.83 Da
• Isoelectric Point: 6.2649

>AT227156 WP_270069186.1 NZ_CP088935:c692892-692653 [Thauera sp. WB-2]
MTSSSQTTVFRSNKTQAVRLPKALELPESVKKVRITAIGNTRIITPAEASWDAWFDGPDVSADFLSSRDQPADQVREAW

Nucleotide

• Length: 240 bp

>AT227156 NZ_CP122727:c2852672-2852304 [Escherichia coli]
GTGTCAGACTCACTCCATGAAACAAATTATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCCTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA