Detailed information of TA system TA227147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3695881..3696537
• Replicon: chromosome
• Accession: NZ_CP088923
• Organism: Rhodanobacter thiooxydans strain FW510-R12

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LRK53_RS16810
• Protein ID: WP_221174208.1
• Coordinates: 3696343..3696537 (+)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LRK53_RS16805
• Protein ID: WP_027492894.1
• Coordinates: 3695881..3696339 (+)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK53_RS16775 (LRK53_16775)	3690988..3691764	-	777	WP_027492889.1	lipid asymmetry maintenance ABC transporter permease subunit MlaE	-
LRK53_RS16780 (LRK53_16780)	3691761..3692597	-	837	WP_027492890.1	ABC transporter ATP-binding protein	-
LRK53_RS16785 (LRK53_16785)	3692676..3693305	-	630	WP_027492891.1	guanylate kinase	-
LRK53_RS16790 (LRK53_16790)	3693302..3694162	-	861	WP_008435059.1	YicC/YloC family endoribonuclease	-
LRK53_RS16795 (LRK53_16795)	3694251..3694979	+	729	WP_027492892.1	ribonuclease PH	-
LRK53_RS16800 (LRK53_16800)	3695076..3695672	+	597	WP_235642396.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
LRK53_RS16805 (LRK53_16805)	3695881..3696339	+	459	WP_027492894.1	helix-turn-helix domain-containing protein	Antitoxin
LRK53_RS16810 (LRK53_16810)	3696343..3696537	+	195	WP_221174208.1	hypothetical protein	Toxin
LRK53_RS16815 (LRK53_16815)	3696537..3696911	+	375	WP_221174209.1	hypothetical protein	-
LRK53_RS16820 (LRK53_16820)	3696939..3698105	+	1167	WP_027492895.1	radical SAM family heme chaperone HemW	-
LRK53_RS16825 (LRK53_16825)	3698194..3700470	+	2277	WP_235642397.1	DUF1631 family protein	-
LRK53_RS16830 (LRK53_16830)	3700467..3700814	+	348	WP_027492896.1	PilZ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3701092..3702222	1130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SinI-like, DNA-binding domain (82-129)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7439.66 Da
• Isoelectric Point: 6.4395

>T227147 WP_221174208.1 NZ_CP088923:3696343-3696537 [Rhodanobacter thiooxydans]
MSNFVAMFDACVLYRESLRNLLLHLALSGLYRARWTARVHEEWIRALCASVPIFPATIWSELDD

Nucleotide

• Length: 195 bp

>T227147 NZ_CP122727:754450-754746 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 16914.37 Da
• Isoelectric Point: 6.2206

>AT227147 WP_027492894.1 NZ_CP088923:3695881-3696339 [Rhodanobacter thiooxydans]
MTALRNEQLVNLPATPQESELARTSSRLLAACIGHGSTARLRVIDGEQEIEVPVAALRMLVDILANMAEGNAMSLVPVHA
ELTTQQAADFLNVSRPYLVGLIDRGELTHHKVGTHRRIYFRDLMAYREQRLMQSHAALDALAEQAQKLDLDY

Nucleotide

• Length: 459 bp

>AT227147 NZ_CP122727:754748-755143 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA