Detailed information of TA system TA227146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1748562..1749157
• Replicon: chromosome
• Accession: NZ_CP088923
• Organism: Rhodanobacter thiooxydans strain FW510-R12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LRK53_RS07835
• Protein ID: WP_027492987.1
• Coordinates: 1748963..1749157 (-)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LRK53_RS07830
• Protein ID: WP_027492988.1
• Coordinates: 1748562..1748966 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK53_RS07815 (LRK53_07815)	1744073..1744306	-	234	WP_235642605.1	hypothetical protein	-
LRK53_RS07820 (LRK53_07820)	1744314..1747916	+	3603	WP_235642655.1	PilC/PilY family type IV pilus protein	-
LRK53_RS07825 (LRK53_07825)	1747983..1748417	+	435	WP_037089839.1	type IV pilin protein	-
LRK53_RS07830 (LRK53_07830)	1748562..1748966	-	405	WP_027492988.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LRK53_RS07835 (LRK53_07835)	1748963..1749157	-	195	WP_027492987.1	type II toxin-antitoxin system HicA family toxin	Toxin
LRK53_RS07840 (LRK53_07840)	1749246..1751234	-	1989	WP_027492986.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7279.49 Da
• Isoelectric Point: 10.4780

>T227146 WP_027492987.1 NZ_CP088923:c1749157-1748963 [Rhodanobacter thiooxydans]
MNSKDLIRELLKDGWILDRINGSHHIYKHPHKAGHLSIPHPEKELGTGLIRQIRKAAGLLKETP

Nucleotide

• Length: 195 bp

>T227146 NZ_CP122727:688639-688953 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14876.77 Da
• Isoelectric Point: 4.3878

>AT227146 WP_027492988.1 NZ_CP088923:c1748966-1748562 [Rhodanobacter thiooxydans]
MKYPIAIETGSETTAWGVAVPDLPGCFSAGDSPDEAYTKAREAIELWLNAALDTGQPIPKAKTLEEHMADPEYKGWAWGV
IEIDLADFDDTVERINITVPKRVLRQIDKYVAVRHENRSGFLARAALEAIYENA

Nucleotide

• Length: 405 bp

>AT227146 NZ_CP122727:688950-689366 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA