Detailed information of TA system TA227144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1392134..1393636
• Replicon: chromosome
• Accession: NZ_CP088923
• Organism: Rhodanobacter thiooxydans strain FW510-R12

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: LRK53_RS06060
• Protein ID: WP_037089858.1
• Coordinates: 1392773..1393636 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: LRK53_RS06055
• Protein ID: WP_027493013.1
• Coordinates: 1392134..1392748 (+)
• Length: 205 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK53_RS06010 (LRK53_06010)	1387525..1387980	-	456	WP_027493018.1	pilin	-
LRK53_RS06015 (LRK53_06015)	1388325..1388768	-	444	WP_027493017.1	pilin	-
LRK53_RS06020 (LRK53_06020)	1388922..1389239	-	318	WP_185754656.1	DUF86 domain-containing protein	-
LRK53_RS06025 (LRK53_06025)	1389308..1389544	-	237	WP_235642561.1	nucleotidyltransferase domain-containing protein	-
LRK53_RS06030 (LRK53_06030)	1389569..1390297	-	729	WP_051257585.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LRK53_RS06035 (LRK53_06035)	1390290..1390760	-	471	WP_235642562.1	helix-turn-helix domain-containing protein	-
LRK53_RS06040 (LRK53_06040)	1390866..1391105	-	240	WP_051257583.1	HigA family addiction module antitoxin	-
LRK53_RS06045 (LRK53_06045)	1391176..1391316	-	141	Protein_1202	type II toxin-antitoxin system RelE/ParE family toxin	-
LRK53_RS06050 (LRK53_06050)	1391480..1391866	-	387	WP_037089861.1	nucleotidyltransferase domain-containing protein	-
LRK53_RS06055 (LRK53_06055)	1392134..1392748	+	615	WP_027493013.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
LRK53_RS06060 (LRK53_06060)	1392773..1393636	+	864	WP_037089858.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LRK53_RS06065 (LRK53_06065)	1393835..1395964	+	2130	WP_027493012.1	S9 family peptidase	-
LRK53_RS06070 (LRK53_06070)	1396666..1397082	+	417	WP_235642563.1	nucleotidyltransferase family protein	-
LRK53_RS06075 (LRK53_06075)	1397702..1398556	-	855	WP_051257627.1	5'-3' exonuclease H3TH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1374734..1397082	22348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-269)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-57)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 31504.29 Da
• Isoelectric Point: 7.6930

>T227144 WP_037089858.1 NZ_CP088923:1392773-1393636 [Rhodanobacter thiooxydans]
MSRNVAASVRARLKNRADATQEDYNLLLTRYGLERLLYRLSVSPHAPNFLLKGALLFLLWHELPHRPTRDADLLGFGDAD
VDPVVAVFRQVCAVACDDGMRFDTRSIRGEVIRKQAGYSGVRIEIQATLDAAVLSLQVDVGFGDVVTPAALAVTYPVLLD
DLPAPQLRAYPKYTVCAEKLQALCVLGMANTRLKDYFDLWLLLGEGSLDTSLLGRAIAATFGRRGTALPDGWPAGLSDLF
ATDAGKQRQWQAFLRKNRLDAPDLADVIRELRTALAQPLDMARGPSA

Nucleotide

• Length: 864 bp

>T227144 NZ_CP122727:226203-226310 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTTGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 205 a.a.
• Molecular weight: 22535.47 Da
• Isoelectric Point: 10.8419

>AT227144 WP_027493013.1 NZ_CP088923:1392134-1392748 [Rhodanobacter thiooxydans]
MTKPPLNHEKQILALARKQPLLRARDLAAHGLPTMTLSRLVAAGKLQRVARGVYSLPQRAISAHRSLAEVAIRVPRGVVC
LLSALRLHGIGTQAPFEVWLAIPQTMPTPRLKQPALRVVRMSGSALSEGIEQVDVDGVAVSVFNLGKTVADCFKYRNKIG
LDVALEALGEAWRARRVTMDELWHYATIDRVHNVMRPYLEALDA

Nucleotide

• Length: 615 bp

>AT227144 NZ_CP122727:c226155-226089 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT