Detailed information of TA system TA227143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1006854..1007656
• Replicon: chromosome
• Accession: NZ_CP088923
• Organism: Rhodanobacter thiooxydans strain FW510-R12

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: LRK53_RS04335
• Protein ID: WP_027492957.1
• Coordinates: 1006854..1007381 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: LRK53_RS04340
• Protein ID: WP_027492958.1
• Coordinates: 1007378..1007656 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK53_RS04320 (LRK53_04320)	1003479..1005308	+	1830	WP_027492955.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
LRK53_RS04325 (LRK53_04325)	1005610..1005894	+	285	WP_027492956.1	type II toxin-antitoxin system ParD family antitoxin	-
LRK53_RS04330 (LRK53_04330)	1005891..1006058	+	168	WP_235642526.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LRK53_RS04335 (LRK53_04335)	1006854..1007381	-	528	WP_027492957.1	hypothetical protein	Toxin
LRK53_RS04340 (LRK53_04340)	1007378..1007656	-	279	WP_027492958.1	DUF1778 domain-containing protein	Antitoxin
LRK53_RS04345 (LRK53_04345)	1007974..1009386	-	1413	WP_027492959.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
LRK53_RS04350 (LRK53_04350)	1009577..1010527	-	951	WP_027492960.1	dTDP-4-dehydrorhamnose reductase	-
LRK53_RS04355 (LRK53_04355)	1010524..1011081	-	558	WP_027492961.1	dTDP-4-dehydrorhamnose 3,5-epimerase	-
LRK53_RS04360 (LRK53_04360)	1011078..1011968	-	891	WP_027492962.1	glucose-1-phosphate thymidylyltransferase RfbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1005891..1035311	29420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18725.55 Da
• Isoelectric Point: 7.9376

>T227143 WP_027492957.1 NZ_CP088923:c1007381-1006854 [Rhodanobacter thiooxydans]
MSVPAWHEEPISKKHDRKLFDCGDSAMNEFLHKYARQSHEAGGAKTFVAVDDADGKTVLGFYSLAPGALAYADTPEQVRK
GLAHHDVPGFRLARIATDKRLQGGGLGGQLLAMAARRCLRAADVVGGVLLMIDAKNGSAAAWYASYGAMTLPDKPLTLVM
ALATFEAELKAKDLL

Nucleotide

• Length: 528 bp

>T227143 NZ_CP122727:c49566-49192 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10158.96 Da
• Isoelectric Point: 11.0037

>AT227143 WP_027492958.1 NZ_CP088923:c1007656-1007378 [Rhodanobacter thiooxydans]
MPRVAVDDNKRMNLRVLPEQKATLVRAAALRHTDLTDFVLQPALREAKAVIAEAERIVLSERDSVAVLRMLENPPAPNAK
LRKAIAALPKQG

Nucleotide

• Length: 279 bp

>AT227143 NZ_CP122727:c50024-49656 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA