Detailed information of TA system TA227140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HTH_37(antitoxin)
• Location: 613392..614020
• Replicon: chromosome
• Accession: NZ_CP088922
• Organism: Rhodanobacter denitrificans strain FW104-10B01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LRK54_RS02865
• Protein ID: WP_007513282.1
• Coordinates: 613844..614020 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LRK54_RS02860
• Protein ID: WP_007513280.1
• Coordinates: 613392..613811 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK54_RS02845 (LRK54_02845)	608834..609844	+	1011	WP_027489896.1	virulence RhuM family protein	-
LRK54_RS02850 (LRK54_02850)	609841..611001	+	1161	WP_231378343.1	AAA family ATPase	-
LRK54_RS02855 (LRK54_02855)	611008..612627	+	1620	WP_027489898.1	type I restriction-modification system subunit M	-
LRK54_RS02860 (LRK54_02860)	613392..613811	-	420	WP_007513280.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LRK54_RS02865 (LRK54_02865)	613844..614020	-	177	WP_007513282.1	type II toxin-antitoxin system HicA family toxin	Toxin
LRK54_RS02870 (LRK54_02870)	614199..614876	+	678	WP_037115012.1	DUF502 domain-containing protein	-
LRK54_RS02875 (LRK54_02875)	614873..616705	+	1833	WP_037115014.1	M14 family metallopeptidase	-
LRK54_RS02880 (LRK54_02880)	616824..618947	+	2124	WP_027489900.1	M13-type metalloendopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6834.93 Da
• Isoelectric Point: 11.0888

>T227140 WP_007513282.1 NZ_CP088922:c614020-613844 [Rhodanobacter denitrificans]
MKYSEFQRWLRQQGVSFEPGKGSHQLARYRGRTSVFPFHGSKEMPEPLRKKILKDLGL

Nucleotide

• Length: 177 bp

>T227140 NZ_CP122724:c6170-5889 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15470.93 Da
• Isoelectric Point: 8.0203

>AT227140 WP_007513280.1 NZ_CP088922:c613811-613392 [Rhodanobacter denitrificans]
MKYPVKLTRADSVVMVTCPDLPEFSSVGDTEGEALREAVDGIETTLQAYMQDRRDIPPPGARKRGQRLVELPPLAVAKLG
LYRAMREQGLRKADLARRLDVHLPQVDRLLDLRHSSKLEQVEAALNALGYRIELAVLAA

Nucleotide

• Length: 420 bp

>AT227140 NZ_CP122724:c6411-6160 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA